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aec chromogen substrate buffer envision™+system, peroxidase (aec) kit  (Agilent technologies)


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    Agilent technologies aec chromogen substrate buffer envision™+system, peroxidase (aec) kit
    Aec Chromogen Substrate Buffer Envision™+System, Peroxidase (Aec) Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aec+buffer+a/pmc01657027-86-6-10
    Average 90 stars, based on 1 article reviews
    aec chromogen substrate buffer envision™+system, peroxidase (aec) kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Melanoma brain metastases that progress on BRAF-MEK inhibitors demonstrate resistance to ipilimumab-nivolumab that is associated with the Innate PD-1 Resistance Signature (IPRES)
    Article Snippet: Slides were stained using a BONDRx autostainer (Leica Biosystems) using the following incubations at room temperature: endogenous peroxidize quenching for 5 min in 3% hydrogen peroxide, protein block for 10 min, primary antibodies for 60 min, mouse or rabbit ImmPRESS (Vector Labs) for 30 min and finally AEC+solution (Dako) for up to 25 min.

    Article Title: Ovarian epithelial cancer: a role for PGE 2 -synthesis and signalling in malignant transformation and progression
    Article Snippet: Primary antibodies against COX-1 (1:100), COX-2 (Cayman Chemical Co., cat. no. 160112, monoclonal, 1:100), mPGES-1 (1:50), EP (1:50) EP 2 (1:50) and CK8 (1:400) diluted in TBS were applied overnight at 4°C, followed by biotinylated goat anti-mouse immunoglobulin (1:60) (VECTASTAIN ABC kit, Vector Laboratories, Inc., Burlingame, CA) for 45 min at 37°C and finally avidin-biotin-peroxidase complex (VECTASTAIN ABC kit, Vector Laboratories) at 37°C for 30 min.

    Article Title: Predictive biomarkers for dasatinib treatment in melanoma
    Article Snippet: On the Autostainer (DAKO) slides were blocked for 10 minutes with 200 μL HRP Block (DAKO).

    Article Title: Increased oxidative stress and severe arterial remodeling induced by permanent high-flow challenge in experimental pulmonary hypertension
    Article Snippet: Staining was completed after incubation with substrate-chromogen AEC solution (Dako ).

    Article Title: Gastrointestinal stromal tumour and hypoglycemia in a Fjord pony: Case report
    Article Snippet: The visualization of the antibody-antigen complex was obtained, after rinsing with TBS, by incubation with horseradish peroxidase-labelled polymer conjugated to goat anti-mouse (vimentin, cytokeratin, desmin, SMA) or goat anti-rabbit (CD117) IgG (kits from Dako Cytomation, Glostrup, Denmark) and a final incubation with ready-to-use AEC+ (3-amino-9ethylcarbazole) substrate-chromogen solution for 10 (SMA), 15 (cytokeratin, vimentin and desmin) or 20 (CD117) minutes. (kits from Dako Cytomation, Glostrup, Denmark).

    Immunohistochemistry:

    Article Title: CD8/CD45RO T-cell infiltration in endoscopic biopsies of colorectal cancer predicts nodal metastasis and survival
    Article Snippet: .. An immunohistochemistry double-stain for pancytokeratin marker AE1/AE3 (Dako, mouse monoclonal, 1:200, enzyme pre-treatment 5 minutes; DAB chromogen) and CD8 (Dako C8/144B, 1:100, pre-treatment with Tris buffer, at 95° for 20 minutes; AEC chromogen) as well as for AE1/AE3 and CD45RO (Abcam, 1:4000; pre-treatment with citrate buffer, at 100° for 30 minutes; AEC chromogen) using an automated Leica Bond III instrument with Haematoxylin counterstaining was performed. ..

    Marker:

    Article Title: CD8/CD45RO T-cell infiltration in endoscopic biopsies of colorectal cancer predicts nodal metastasis and survival
    Article Snippet: .. An immunohistochemistry double-stain for pancytokeratin marker AE1/AE3 (Dako, mouse monoclonal, 1:200, enzyme pre-treatment 5 minutes; DAB chromogen) and CD8 (Dako C8/144B, 1:100, pre-treatment with Tris buffer, at 95° for 20 minutes; AEC chromogen) as well as for AE1/AE3 and CD45RO (Abcam, 1:4000; pre-treatment with citrate buffer, at 100° for 30 minutes; AEC chromogen) using an automated Leica Bond III instrument with Haematoxylin counterstaining was performed. ..

    Automated:

    Article Title: CD8/CD45RO T-cell infiltration in endoscopic biopsies of colorectal cancer predicts nodal metastasis and survival
    Article Snippet: .. An immunohistochemistry double-stain for pancytokeratin marker AE1/AE3 (Dako, mouse monoclonal, 1:200, enzyme pre-treatment 5 minutes; DAB chromogen) and CD8 (Dako C8/144B, 1:100, pre-treatment with Tris buffer, at 95° for 20 minutes; AEC chromogen) as well as for AE1/AE3 and CD45RO (Abcam, 1:4000; pre-treatment with citrate buffer, at 100° for 30 minutes; AEC chromogen) using an automated Leica Bond III instrument with Haematoxylin counterstaining was performed. ..

    Blocking Assay:

    Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.
    Article Snippet: .. After blocking with 5% bovine serum albumin in TBST at room temperature for 1 h, 2 μm tissue sections were incubated with megalin (sc-515772, Santa Cruz, 1: 100), amnionless (sc-365384, Santa Cruz, 1:50), and albumin (16475-1-AP, Proteintech) antibodies overnight at 4°C, followed by incubation with the Biotin-SP AffiniPure Goat Anti-Mouse (Yeasen, 33203ES60,1: 400) secondary antibody for 1 h. After washing, the slides were incubated with streptavidin solution (Yeasen, 35105ES60, 1:300) for 30 min. AEC working solution (DAKO, K346111-2) was applied to cover the tissue section completely, and color development was monitored at room temperature using an Olympus DP27 microscope. ..

    Incubation:

    Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.
    Article Snippet: .. After blocking with 5% bovine serum albumin in TBST at room temperature for 1 h, 2 μm tissue sections were incubated with megalin (sc-515772, Santa Cruz, 1: 100), amnionless (sc-365384, Santa Cruz, 1:50), and albumin (16475-1-AP, Proteintech) antibodies overnight at 4°C, followed by incubation with the Biotin-SP AffiniPure Goat Anti-Mouse (Yeasen, 33203ES60,1: 400) secondary antibody for 1 h. After washing, the slides were incubated with streptavidin solution (Yeasen, 35105ES60, 1:300) for 30 min. AEC working solution (DAKO, K346111-2) was applied to cover the tissue section completely, and color development was monitored at room temperature using an Olympus DP27 microscope. ..

    Microscopy:

    Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.
    Article Snippet: .. After blocking with 5% bovine serum albumin in TBST at room temperature for 1 h, 2 μm tissue sections were incubated with megalin (sc-515772, Santa Cruz, 1: 100), amnionless (sc-365384, Santa Cruz, 1:50), and albumin (16475-1-AP, Proteintech) antibodies overnight at 4°C, followed by incubation with the Biotin-SP AffiniPure Goat Anti-Mouse (Yeasen, 33203ES60,1: 400) secondary antibody for 1 h. After washing, the slides were incubated with streptavidin solution (Yeasen, 35105ES60, 1:300) for 30 min. AEC working solution (DAKO, K346111-2) was applied to cover the tissue section completely, and color development was monitored at room temperature using an Olympus DP27 microscope. ..



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